|
KMID : 0545120070170121970
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 12 p.1970 ~ p.1975
|
|
|
Isolation and Structural Determination of Squalene Synthase Inhibitor from Prunus mume Fruit
|
|
Choi Sung-Won
Hur Nam-Yoon
Ahn Soon-Cheol
Kim Dong-Seob
Lee Jae-Kwon
Kim Dae-Ok
Park Seung-Kook
Kim Byung-Yong
Baik Moo-Yeol
|
|
|
Abstract
|
|
|
|
Squalene synthase plays an important role in the cholesterol biosynthetic pathway. Inhibiting this enzyme in hypercholesterolemia can lower not only plasma cholesterol but also plasma triglyceride levels. A squalene synthase inhibitor was screened from Prunus mume fruit, and then purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. The squalene synthase inhibitor was identified as chlorogenic acid with a molecular mass of 354 Da and a molecular formula of C16H18O9 based on UV spectrophotometry, 1H and 13C NMRs, and mass spectrometry. Chlorogenic acid inhibited the squalene synthase of pig liver with an IC50 level of 100 nM. Since chlorogenic acid was an effective inhibitor against the squalene synthase of an animal source, it may be a potential therapeutic agent for hypercholesterolemia.
|
|
|
KEYWORD
|
|
|
Squalene synthase inhibitor, chlorogenic acid, Prunus mume, hypercholesterolemia
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|